Dr. Hanan A. Nour El-Din

B.Sc. in Botany, Botany Department, Faculty of Science,

Cairo University, 1991

M.Sc. in Molecular Biology, Botany Department, Faculty of Science,

Cairo University, 1997

Ph.D in Molecular Biology, Microbiology Department, Faculty of Science,

Ain Shams University, 2003

Researcher (Lecturer) of Molecular Virology, Molecular Plant Pathogenesis Laboratory (MPPL), Agricultural Genetic Engineering Research Institute (AGERI), Agricultural Research Centre (ARC), Giza.

Senior Researcher (Associate Professor) of Molecular Virology, Molecular Plant Pathogenesis Laboratory (MPPL), Agricultural Genetic Engineering Research Institute (AGERI), Agricultural Research Centre (ARC), Giza

Head of Microbial Molecular Biology Department

Senior (Professor) of Molecular Virology, Molecular Plant Pathogenesis Laboratory (MPPL), Agricultural Genetic Engineering Research Institute (AGERI), Agricultural Research Centre (ARC), Giza

Jan 2015


Personal Details                                  

Name: Hanan Abd El-Raheim Nour El-Din Mohamed
Address: AGERI, ARC, 9 Gamaa street, Giza 12619, Egypt
Tel: 016 5350480
Mobile: 0100 6610042
E-mail:

This email address is being protected from spambots. You need JavaScript enabled to view it.

Nationality: Egyptian

Professional Degrees:

B.Sc. in Botany, Botany Department, Faculty of Science, Cairo University, 1991

M.Sc. in Molecular Biology, Botany Department, Faculty of Science, Cairo University, 1997.

Ph.D in Molecular Biology, Microbiology Department, Faculty of Science, Ain Shams University, 2003.

Specialist: Molecular Virologist

Current Position:

Head of Microbial Molecular Biology Department and Professor of Molecular Virology, Molecular Plant Pathogenesis Laboratory (MPPL), Agricultural Genetic Engineering Research Institute (AGERI), Agricultural Research Centre (ARC), Giza.


Job History:

 

Research Assistant From Nov. 1992   to May 1997
Assistant Researcher From May 1997 to Dec 2003.
Researcher From Jun 2004 to 2009.

Senior assistant

Senior (Professor)

Head of Microbial Molecular Biology

Department

From April 2009 to 2014.

From May 2014 till now.

From September 2014.

I obtained my B.Sc. in Botany from Cairo University in 1991. In 1992, I successfully passed the post-graduate courses as a partial fulfillment the requirements of the Master degree. I started my scientific career in the Agricultural Genetic Engineering Research Institute (AGERI) in Egypt. I’m working at Molecular Plant Pathogenesis laboratory. I’m working on plant diseases. My M.Sc. project was concerning with the genetic variation within the three different species of Ascochyta pathogens. In 1997, my institute was in charge of the evaluation of the purity of maize inbred lines that are cultivated in Egypt. I shared in this mission by using the isozymes.

I have extensive training and experience in the following techniques:

  • Isolation, purification and identification of plant pathogens.
  • DNA and RNA isolation and purification.
  • Restriction digestion analysis.
  • Polymerase chain reaction (PCR)
  • RAPD-PCR.
  • Southern blotting.
  • Western blotting
  • Protein expression in E. coli.
  • Production of ELISA diagnostic kit for bacterial and viral detection.

My Ph.D project was concerning with cloning the coat protein gene of an Egyptian isolate of a banana virus and then produce specific polyclonal antibodies which led to production of an ELISA kit for virus detection.

         

Attendance of the training courses:

  • September 10th – 21st, 1995: Regional training course on Diagnosis of plant viruses. organized by ICARDA at AGERI.
  • Diagnosis of Plant Viruses with Special Emphasis on Faba Bean Necrotic Yellows Virus.

Teaching courses:

  1. Diagnostic of genetically modified plants using molecular markers and ELISA (International) in Agricultural Genetic Engineering Research Institute (AGERI). From 1-26 Sep. 2002.
  1. Plant Tissue Culture and Transformation Techniques. In Agricultural Genetic Engineering Research Institute (AGERI). From 1-13 April. 2005.
  1. Training course on Serological detection of transgenic plant by ELISA. For Undergraduate Students, Faculty of Agriculture, Cairo university from 19-7 to 2-8/ 2005 in Agricultural Genetic Engineering Research Institute (AGERI).
  1. Training course on Molecular and Transformation Techniques For Undergraduate Students, Faculty of Agriculture, Cairo University from 10 to 21 August, 2008.
  1. Training course on Serological detection of virus by ELISA test for Undergraduate students, faculty of Agriculture, Cairo university from 19-30 August, 2009 in Agricultural Genetic

     Engineering Research Institute (AGERI).

  1. Training course on Basic of Agricultural Biotechnology for Undergraduate students, faculty of Agriculture, Cairo University from 2-14 August, 2009 in Agricultural Genetic Engineering Research Institute (AGERI).
  1. Training course on Basic of Agricultural Biotechnology (Serological detection of plant viruses) for Undergraduate students, faculty of Agriculture, Cairo University from 04-15 and18-29 July 2010 in Agricultural Genetic Engineering Research Institute (AGERI).
  1. Training course on Biotechnology techniques (Principals and applications of serological methods) for Undergraduate students, faculty of Agriculture, Cairo University from 17-28 July 2011 in Agricultural Genetic Engineering Research Institute (AGERI).
  1. Training course on Biotechnology techniques (Principals and applications of serological methods) for Undergraduate students, faculty of Agriculture, Cairo University from 5-16, August 2012 in Agricultural Genetic Engineering Research Institute (AGERI).
  1. Training course on Serological and novel methodologies for detection of GMO plant. Organized by AOAD and AGERI, From 2-12 Dec. 2012.
  1. Training course on Biotechnological Techniques for Undergraduate students, faculty of Agriculture and faculty of Science, Cairo University from 8-19, September 2013 in Agricultural Genetic Engineering Research Institute (AGERI).
  1. Training course on Principals and applications of serological methods for Undergraduate students, faculty of Agriculture, Cairo University from 10-21, Augaust 2014 in Agricultural Genetic Engineering Research Institute (AGERI).
  1. Training course on Biotechnological Techniques of Serological Methods for Undergraduate students, faculty of Agriculture, Mansoura University from 8-12, February 2015 in Agricultural Genetic Engineering Research Institute (AGERI).
  1. Training course on Biotechnology techniques (Principals and applications of serological methods) for Undergraduate students, faculty of Agriculture, Cairo University from 4-13, August 2015 in Agricultural Genetic Engineering Research Institute (AGERI).
  1. Training course on Biotechnology techniques (Principals and applications of serological methods) for Undergraduate students, faculty of Science, Cairo University from 18-31, August 2015 in Agricultural Genetic Engineering Research Institute (AGERI).

Projects:

I have participated in the titled project:

1- Improvement of diagnostic tools for the certification of virus free strawberry proposal materials (From 2004 to 2006). Funded by AERI Institutional Linkage Project, Midwest Universities Consortium for International Activities (MUCIA), Inc. and university of Illinois.

 

2-    Production of polyclonal and/or monoclonal antibodies for preparation of ELIZA kits (As PI from 2009 to 2012), funded from STDF (Science Technology and Development Fund). PI

 

3-    Improvement of Diagnostic Tools for some grapevine viruses in Egypt (In research team as assistant professor from 2009 to 2011), funded from STDF (Science Technology and Development Fund). Researsh team.

4-    Preparation of diagnostic ELISA kit(s) for Some bacterial toxins by radiation and molecular technique from   to   , funded from STDF (Science Technology and Development Fund). Co.PI

Symposiums:

  1. Symposium on trends in biotechnology research and application, AGERI/AERI. Institutional linkage project. Midwest universities consortium for international activities (MUCIA) Inc., university of Illonois, Jan. 11-2004.
  1. Symposium on Commercialization and Utilization of Biotechnology .   AGERI/ABSP Collaborative project, May 29-Jun, 2000.

Conferences:

  1. US-Egypt Workshop on Genetic Engineering and Genomics. 5-6 December, 2003. Mubarak City for Scientific Research and Technology Applications, New Borg El-Arab. (Poster)
  2. The international conference of Genetic engineering and its application towards safe genetically modified products for Man and his environment. At Sharm El-Sheik city, South Sinia, Egypt, 2-5 April 2004.
  1. The third international Conferance on Scientific research and technology development in Arab world. April from 11to 14-2004 in Riyadh. (Speaker)
  1. The international conference of Women in Science. At Alexandria, Bibliotheca Alexandria, Egypt, 23-24 October 2007.
  1. The international conference under the auspicious patronage ofthe RoyalScientific Society, the Arab Science and Technology Foundation is organizing a conference titled: Resaerch, Development and Innovation: Biotechnology in the Arab World, with the collaboration of Pacific Northwest National Laboratory,s (PNNL) and the Arab Authority for Agricultural Investment and Development (AAAID). Jordan – Amman: 03- 05 March 2008. (Speaker)
  1. The international Biovision Alexandria 2008 of New life science: from promises to practice. At Alexandria, Bibliotheca Alexandria, Egypt, 12-16 April 2008.
  1. The First international Conference for Applications of Biotechnology organized by Faculty of Biotechnology October University for Modern Science and Arts and The University of Greenwich at 18-19 October, 2008.
  1. The first international conference on biotechnology, organized by King Soad university, Riyadh, Saudia Arabia from 12-18 Feb 2009 (Poster).
  1. The second international conference for applications of biotechnology

10- The 12 th IAPB conference for International Association for Plant Biotechnology conference, in St.Louis\Missouri at June 6-11, 2010.

11- The 3rd international conference of virology for Current    Challenges of Viral Diseases organizedby Egyptian Socity of Virology, In Cairo University conference center at 24-25 November, 2010.

Seminars:

Genetically Modified Food. April 17-2005 in Agricultural Research Center     (ARC), Agricultural Genetic Engineering Research Institute (AGERI).

Workshops:

  1. Workshop on Environmental risk of Genetic Modified Crops organized by AGERI and Monsanto Co. USA. May 2001.
  1. Workshop on The Application of Regulatory Sciences in the Evaluation of Health and Environmental Risk of Genetically Modified Crops. Orgaized by AGERI, ARC Egypt and Monsanto Co. USA. May 23, 2001,Cairo, Egypt.
  1. Regional workshop on Biosafety Development, 15-18      April 2002 at AGERI, ARC. Sponsored by AGERI, USAD, ICARDA and ISESCO.
  1. Workshop on Biotechnology in Egypt, 19 July, 2004 organized by AGERI and EPIC.
  1. Workshop on Implications of Regulatory Sciences in Developing Insect Resistant Cotton, 8-9 Febreuary, 2006 organized by AGERI and EPIC.
  1. 6.Workshop on Better Plants for Better Life, 24th April 2007 organized by AGERI, ARC and FAL (Bundesforschungsanstalt FÜr Landwirtschaft-Germany). Egyptian-German year of Science and Technology 2007.

  1. Workshop on Biotechnology for improving agricultural crops

1- Supervision on the scientific Thesis:

Master Degree:

  1. 1.Iman Shahwan moheb El-Din Shahwan, 2007.

Studies on some Egyptian Faba bean viruses, Banhauniversity, Faculty of Agriculture.

Ph.D Degree:

  1. 1.Shaimaa Mohamed Abd El-salam 2008.

Molecular studies on Banana bunchy Top Virus, Ain Shams University-Girl collouge, Faculty of Science, Department of Botany.

  1. 2.Shimaa Abdel wahab Abdel sadyek Mohamed 2012.

Studying the efficiency of certain natural compounds against some phytopathogenic fungi. Pesticides Formulation Department, Central Agricultural Pesticides Laboratory, Agricultural Research Center.

  1. 3.Mohamed Nagah Abu El-Naga 2013. Ain Shams University, Faculty of Science, Department of Microbiology. Stimulation of Staphylococcus aureus Enterotoxins production using radiation and molecular techniques for preparation its ELISA kits.
  2. 4.Mohamed Talal

2- External Examiner for the academic semester (2013) in the faculty of biotechnology, October University for Modern Sciences and Arts (MSA).    

Publications

1- A.M. Tohamy, H.A. Nour Eldin, Z.K. Mohamed, Gh.A. Gad El-Karim and M.A. Madkour (1997). Identification of three species of Ascochyta spp. By random amplified polymorphic DNA. Phytopathology 87(6) (Supplement), 1997 S97. Publication no. P-1997-0694-AMA.

2-H.A. Nour Eldin, M.I. Salama, A.B. Barakat, A.M. Saleim and A.S. Sadik (2005). Nucleotide sequence of BBTV-cp gene and use its fusion protein induced via QIAexpress system for producing specific polyclonal antibodies. Arab Journal of Biotechnology Volume 8, Number 2.

3-Roba M. Ismail, Sh.F. El-Kholy, H.A. Nour Eldin, A.S. Sadik, R.M. Harding, A.Z.E. Abdelsalam and Hanaiya A. ElItriby (2005). Production of transgenic banana plants containing a different gene. The second symposium on tissue culture and its application in Arab agricultural investments. United Arab Imarets, 12-14 June 2005.

4-M.A. Abdel-wahid, F. G. Rawia, H.A. Nour El-Din, M.O. Wasfy and A.S. Sadik (2005). Microbiological, molecular and serological studies on Mycobacterium tuberculosis complex from animal and human sources. Ain Shams University, Faculty of Agriculture, Research Bulletin.

5-A.I. Abdel-fatah, H.A. Nour El-Din, A. Abodooh and A.s. sadik (2005). Occurance of two sugarcane mosaic potyvirus (SCMV) strains in sugarcane.Third International Symposium on Biotechnology December 15 - 18, 2005. Institute of Biotechnology and Genetic Engineering, University of Sindh, Jamshoro, Pakistan.

6-Roba M. Ismail, Hanan A. Nour El-Din, Ismail A. Ismail and Atef S. Sadik (2007). Production of transgenic banana plants expressing the CMV-cp gene. The second international conference of "Genetic engineering and its Applications" organized by the Egyptian Society of Genetics, hold in Sharma El-Sheikh, south Sinai, Egypt, 14-17 November, 2006.

7-Khalil S.M., M.I. Salama, I.A. Abdel-Hamid, Hanan A. Nour El-Din and A.S.Sadik (2007). Effective detection of banana bunchy top nanovirus using DNA probs. Pakistanian Journal of Biotechnology, Volume 4 (1-2) 113-118.

8-M.I. Salama, S.M. Khalil, I.A. Abdel-Hamid, Hanan A. Nour El-Din and A.S. sadik. 2007. Partial nucleotide sequence of segment one encoding replicase gene of banana bunchy top nanovirus. The Egyptian Society of Genetics Vol. 36.

9-Nour El-Din A. Hanan, Abdel-salam M.; Hewedy A. Maha; and M.I. Salama (2008). Nucleotide sequences of segment five of the Egyptian banana bunchy top babuvirus. Egyptian Journal of Genetics and cytology, volume 37, No. 2, 209-221.

10-Salama, M.I.; El-Domyati, F.M.; Salem, R.E.; Nour El-Din, Hanan and Sadik, A.S (2007). Production of polyclonal antibodies specific to Citrus Tristeza Virus (CTV) using the 6X His-tagged fusion protein. Pakistan Journal of Biotechnology, Volume 4 No.1-2.

11-Mahdy, A.M.M.; R.N. Fawzy; M.A. Hafez; Hanan A. Nour El-Din;and Eman S.M. Shahwan. (2007). Inducing systemic resistance against Bean Yellow mosaic Potyvirus using botanical extracts. Egyptian Journal Of Virology, Volume 4, 129-145.

12-Salem, R. E.; Fatma Badawy; Salama, M. I.; Hanan A. Nour El-Din; El-Dougdoug, KH. A. and Eman Fahmy. Detection of Citrus Psorosis Ophiovirus in citrus CV. Navel orange in Egypt (2010). Egyptian Journal of Genetics Cytology Vol. 39: 305-314, July, 2010.

13- Hanan A. Nour El-Din, S. Khalil and Shimaa M. Abdelsalam. Molecular characterization and phylogenetic analysis studies of component six of Banana bunchy top babuvirus (BBTV DNA-6) (2011). Egyptian Journal of Genetics and Cytology, Vol.40: 99-111.

14-El-Shiekh, Y.W. A.1; Nour El-Din, A. Hanan, Mohamed, A. A. Shaymaa and Karam EL-Din, A. Zahraa. Antifungal activity of some Naturally Occurring Compounds against Economically Important Phytopathogic Fungi. Nature and Science 2012,10(6):114-123 (ISSN: 1545-0740).

15-Bakr, Y. M.; Hanan A. Nour El-Din;. A; Abd El-Wahed, W. F. and Haemeida, A. A. Application of epitopes prediction for antibodies production against potato leaf roll virus (2012). International Journal of Virology 8(1):121-127.

16-Hanan A. Nour El-Din; R. Salem and K. S. Abdallah. Cloning and characterization of cucumber mosaic virus coat protein gene from infected banana plants in Egypt. Egyptian journal of genetics and cytology 42:151-161, January, 2013.

17-Khaled S. Abdallah and Hanan A. Nour El-Din. Development of latex agglutination test for rapid detection of Citrus Tristeza Closterovirus.Egyptian journal of genetics and cytology 42:223-231, July, 2013

18- Hanan A. Nour El-Din. Immunological comparisonsof polyclonal antibodies produced against the recombinant coat protein and viral particles for detecting Egyptian Banana- cucumber mosaic cucumovirus.Egyptian journal of genetics and cytology 42:259-267, July, 2013

19- Reda E. salem,M. I. Salama and Hanan A. Nour El-Din.Expression of Cucumber Mosaic virus coat protein for the production of recombinant polyclonal antibodies. Arab Journal of Biotechnology.Vol.16, No.2. July 2013.

20- HebaH Abouseadaa,Gamal H Osman,Ahmed M Ramadan,Sameh Hassanein,Mohamed T Abdelsattar,Yasser B Morsy,Hussien F Alameldin,Doaa K El-Ghareeb, Hanan A Nour-Eldin,Reda Salem,Adel A Gad,Soheir E Elkhodary,Maher M Shehata, Hala M Mahfouz,Hala F EissaandAhmed Bahieldin.Development of transgenic wheat (Triticum aestivum L.) expressing avidin gene conferring resistance to stored product insects. BMC Plant Biology  15:183 2015.

References:

  • Professor of Agricultural Virology, Department of Agric. Microbiology, Faculty of Agriculture, Ain Shams University. This email address is being protected from spambots. You need JavaScript enabled to view it.
  • , Ph.D. Plant virologist. Senior in MPPL in AGERI. ARC, 9 Gamaa St., 12619, Giza Egypt. This email address is being protected from spambots. You need JavaScript enabled to view it.

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